A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs.
نویسندگان
چکیده
We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which express either Cre- or FLP-recombinase. Plasmids containing authentic recognition targets for either recombinase (loxPs or FRTs) are recombined when propagated in the appropriate strain. 294-Cre and 294-FLP thus provide a simple test for the recombination competence of constructs that are designed for use in Cre- or FLP-mediated genomic manipulations.
منابع مشابه
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 24 15 شماره
صفحات -
تاریخ انتشار 1996